Identification of potyviruses using the polymerase chain reaction with degenerate primers

Identification of potyviruses using the polymerase chain reaction with degenerate primers.

Local areas of conserved amino acid sequence in the replicase and coat proteins of potyviruses were used to select nucleotide sequences for use in the construction of sets of degenerate oligonucleotide primers for amplification of DNA fragments on potyvirus-specific templates in a combined assay of reverse transcription and the polymerase chain reaction (RT-PCR). Sequences selected for the cons...

Degenerate Primers Facilitate the Detection and Identification of Potyviruses From the Northwest Region of Iran

Background: Potyviruses are accounted for 40% of viral diseases of various crops including vegetables and legumes. Potyviruses can be transmitted through plant sap, seeds and many aphid species. Due to ease of spread of these viruses detection of such viruses are crucial as to the control of the incited diseases. Objectives: This study compared the efficiencies of two couples of primers in the...

Application of specific primers and polymerase chain reaction for identification of ralstonia solanacearum biovar2

Characterization of superoxide dismutase Gram-positive bacteria by polymerase chain degenerate primers genes from reaction using

An internal fragment representing approximately 85% of sod genes from seven Gram-positive bacteria was amplified by using degenerate primers in a polymerase chain reaction assay. The DNA sequences of sod polymerase chain reaction products from Clostridium perfringens, Enterococcus faecalis, Enterococcus faecium, Lactococcus lactis, Staphylococcus aureus, Streptococcus agalactiae, Streptococcus ...

Using the polymerase chain reaction with degenerate primers, three new members of the hsp70 gene family of Trypanosoma brucei

African trypanosomes, parasitic protozoa of the Order Kinetoplastida, are the causative agents of sleeping sickness in humans and nagana in cattle. As judged by 18 S rRNA sequence homology (Sogin et al., 1989), these organisms are among the most primitive of the eukaryotic kingdom, being as distant evolutionarily from yeast as they are from vertebrates. Nevertheless, trypanosomes have provided ...